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. 2021 Apr 22;10(9):e019177. doi: 10.1161/JAHA.120.019177

Figure 4. Polyethylene glycol‐20k and MCC950 upregulated the expression of SIRT1 (sirtuin 1)/PGC1‐α (peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha) in tandem with inhibition of NLRP3 (the NOD‐like receptor family protein 3) inflammasomes.

Figure 4

A, Representative western blot analysis of SIRT1/PGC1‐α and NLRP3 inflammasomes pathway; (B), Relative expression of SIRT1; (C), Relative expression of PGC‐1α; (D), Relative expression of NLRP3; (E), Relative expression of cleaved‐caspase‐1/pro‐caspase‐1; (F), Relative expression of ASC; (G), Relative expression of GSDMD; (H), Relative expression of interleukin‐1β; (I), plasma IL‐1β concentration. Relative expression values were normalized to GAPDH internal control. C indicates cardiac arrest control group; GSDMD, gasdermin D; IL‐1β, interleukin‐1β; NLRP3, the NOD‐like receptor family protein 3; M, the MCC950 group; P, polyethylene glycol‐20k group; P&M, the polyethylene glycol‐20k group‒MCC950 group; PGC1‐α, peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha; ASC, apoptosis‐associated speck‐like protein; S, the sham group; and SIRT1, sirtuin 1. *P<0.05 vs S, # P<0.05 vs C. All statistical analysis was performed by 1‐way ANOVA, followed by Tukey post‐hoc analysis (n=6 for each group). Data are expressed as mean±SD.