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. 2021 Apr 29;10(9):e018062. doi: 10.1161/JAHA.120.018062

Figure 12. Relationship among linc01278 and miR‐500b‐5p as well as ACTG2 in vascular smooth muscle cells activation in vitro.

Figure 12

A, linc01278 probable targeted binding site of 3'‐untranslated region of miR‐500b‐5p. B, miR‐500b‐5p probable targeted binding site of 3'‐untranslated region of ACTG2. The vertical lines represented binding sites. C and D, Luciferase assay of wild‐type and mutant linc01278 and ACTG2 3' untranslated region in miR‐500b‐5p mimics and miR‐500b‐5p mimics control into 293T cells. (n=6). *,# P<0.05. E, ACTG2 downexpression in aortic dissection tissue, Si‐linc01278 and Si‐linc01278+miR‐500b‐5p inhibitor control, Ov‐linc01278 control, and Ov‐linc01278+miR‐500b‐5p mimic transfected vascular smooth muscle cells as determined by quantitative reverse transcription‐PCR. *,#,& P<0.05. F, Western blot analysis of ACTG2 in tissues and vascular smooth muscle cells. AD indicates aortic dissection; and PCR, polymerase chain reaction.