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. 2021 Jun 2;22(2):824. doi: 10.3892/etm.2021.10256

Figure 4.

Figure 4

miR-133a-3p regulates intestinal epithelial cells by targeting TAGLN2 expression. (A) The predicted target gene sequence of miR-133a-3p and the binding TAGLN2 3'-UTR. (B) Relative luciferase activity of different groups. (C) Western blot analysis results of TGALN2 in transfected cells (miR-133a-3p mimics, NC mimics, miR-133a-3p inhibitor and NC inhibitor). (D) Cell Counting kit-8 assay results of the TAGLN2 siRNA and control groups. (E) Apoptotic cells in TAGLN2 siRNA and control group. (F) Western blot analysis results of apoptosis-related proteins Bax and Bcl2 in the TAGLN2 siRNA and control groups. **P<0.01; *P<0.05. miR, microRNA; TAGLN2, transgelin-2; UTR, untranslated region; siRNA, small interfering RNA.