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. 2021 Jun 4;22(2):835. doi: 10.3892/etm.2021.10267

Figure 2.

Figure 2

ZFAS1-knockdown inhibits the ox-LDL-induced excessive proliferation of VSMCs. (A) Relative mRNA ZFAS1 expression was quantified via reverse transcription-quantitative PCR following ZFAS1-knockdown. (B) Viability of VSMCs was examined using the Cell Counting Kit-8 assay. (C) Protein expression levels of Ki67 and PCNA were determined by western blotting. The data are expressed as the mean ± SEM from three independent experiments. ***P<0.001 vs. Control; #P<0.05 and ###P<0.001 vs. ox-LDL + si-NC. ZFAS1, zinc finger antisense 1; ox-LDL, oxidized low-density lipoprotein; VSMCs, vascular smooth muscle cells; PCNA, proliferating cell nuclear antigen; si, small interfering RNA; NC, negative control.