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. 2021 May 31;11:638311. doi: 10.3389/fonc.2021.638311

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Copyright © 2021 Huang, Liao, Hsu, Chan, Liao, Yang, Chen, Sung and Tsai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of ENO1 on the cell-surface of a subpopulation of CSCs. (A) Representative FACS plots showing patterns of CD44, CD133, and surface ENO1 (sENO1) staining of primary prostate adenocarcinoma (PAC)-derived 22Rv-1 cells with the frequency of the boxed CD44⁺CD133⁺ cell population (representing CSCs in PAC; left) or sENO1⁺ cells in CD44⁺CD133⁺ CSCs (middle) or cells in the other subpopulations (representing non-CSCs; right) shown. (B) The percentages of sENO1⁺ cell subpopulation in CD44⁺CD133⁺ 22Rv-1 cells or cells in the other subpopulations (others). (C) The percentages of sENO1⁺ cell subpopulation in CD44⁺CD133⁺ PC-3 cells or cells in the other subpopulations. (D) The percentages of sENO1⁺ cell subpopulation in CD90⁺ gastric adenocarcinoma (GAC) AGS or NCI-N87 cells (representing CSCs in GAC) or CD90- cells (representing non-CSCs). Error bars represent mean ± SEM from three independent experiment (n = 3). Unpaired t-test was performed throughout where **p < 0.01; ***p < 0.001 in (B–D).