Figure 3.

TRIM7 was targeted by miR-4491 in non-small cell lung cancer cell lines. (A) Target genes of miR-4491. (B) Complementary sites between TRIM7 and miR-4491. (C and D) miR-4491 inhibitor significantly increased TRIM7 expression level and (E) decreased p65 protein expression in A549 and NCI-H1650 cells. miR-4491 mimic significantly decreased the relative luciferase activity of (F) TRIM7 wild-type but not (G) TRIM7 mutant variants in A549 and NCI-H1650 cells. *P<0.05, **P<0.01 and ***P<0.001 vs. miR-NC mimic or miR-NC inhibitor. TRIM7, tripartite motif containing 7; miR, microRNA; NC, negative control.