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. 2021 Jun 7;22(11):6137. doi: 10.3390/ijms22116137

Figure 8.

Figure 8

Effects of Pangasius hypophthalmus fish skin hydrolysates (fsCH) on transepidermal water loss (TEWL), skin hydration, and aquaporin 3 (AQP3) level in ultraviolet-B (UV-B) radiation-exposed mice. SKH-1 hairless mice were exposed to UV-B radiation three times a week and treated with 206 mg/kg N-acetyl-D-glucosamine (NAG), 15 mg/kg glycine-proline-hydroxyproline tripeptide (GPH), and 206–412 mg/kg fsCH orally for eight weeks. TEWL (A), skin hydration (B), and TEWL/skin hydration ratio (C) were measured and calculated. The AQP3 level of skin tissues was immunohistochemically confirmed by FITC-green staining (D). Skin tissue extracts were electrophoresed on 10% SDS-PAGE and subjected to Western blot analysis with a primary antibody against AQP3 (E). β-Actin antibody was used as an internal control. The bar graph (mean ± SEM, n = 3) in the right panel represents the quantitative results of blot band on the left obtained from a densitometer. Respective values in bar graphs not sharing a small letter are different at p < 0.05.