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. 2021 Jun 5;22(11):6105. doi: 10.3390/ijms22116105

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Histological analysis of Cx43 expression in mouse ventricles. (A) Immunofluorescence of Cx43 confocal image in the LV (left panel) and the RV (right panel) among the study groups (n = 5 for each group). Nuclei with DAPI staining appear blue. The plasma membrane with WGA staining appears red, and green for Cx43-positive staining. The scale bars indicate 10 μm. (B)The quantification of Cx43 signaling integrated from a series of confocal image sections in ventricles among the study groups. (C) IHC staining of Cx43 in mouse ventricles of each group. Blue is used for cell nucleus staining and brown for Cx43-positive staining. *: p < 0.05 compared with the control group; #: p < 0.05 compared with the MS group; $: p < 0.05 compared with the EMPA group.