Figure 1.

The Proactive Molecular Risk Classifier for Endometrial cancer (ProMisE) molecular classification algorithm [21,22,23]. Our final cohort included 58 tumors with sufficient tissue for testing. Mismatch repair (MMR) status was evaluated by immunohistochemistry targeting of proteins MLH1, PMS2, MSH2, and MSH6, and tumors were categorized as MMR-deficient if they exhibited loss of any of these proteins. MMR-intact tumors were then evaluated for mutations in the exonuclease domain of the polymerase-ε gene (POLE) via single gene sequencing. Tumors with wild-type POLE sequencing were then subjected to IHC for p53, which identified 4 tumors with strong and diffuse expression of p53. Confirmatory p53 sequencing was performed on these 4 samples to ensure accuracy of IHC. Abbreviations: EC, endometrioid endometrial adenocarcinoma; EIN, endometrial intraepithelial neoplasia.