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. 2021 Jun 5;22(11):6096. doi: 10.3390/ijms22116096

Figure 2.

Figure 2

Inhibitory effect of carnosic acid on the translation of Nox4 enzyme components in MDI-induced differentiation of 3T3-L1 pre-adipocytes to adipocytes for two days. (A) Translation of Nox4, p47phox, and p22phox as determined by Western blotting analysis after differentiation for two days. (B) Graphic representation of densitometric analysis of Nox4 blot. (C) Graphic representation of densitometric analysis of p47phox blot. (D) Graphic representation of densitometric analysis of p22phox blot. Data are presented as averages of three independent experiments performed in triplicates and expressed as percentages of the value of control (means ± standard error mean, n = 3). ###: p < 0.001 vs. Undiff. Different corresponding letters indicate significant differences at p < 0.05 using Duncan’s test. (Undiff: undifferentiated, which was not treated with the MDI hormone mixture; C: control, which was treated with the MDI hormone mixture).