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. 2021 Jun 5;22(11):6096. doi: 10.3390/ijms22116096

Figure 3.

Figure 3

Inhibitory effect of carnosic acid on translation and phosphorylation of NF-κB and IκBα, and translocation of NF-κB into the nucleus during the differentiation of MDI-induced 3T3-L1 pre-adipocytes into adipocytes for two days. (A) Translated and phosphorylated NF-κB and IκBα in whole cells by Western blotting analysis after differentiation for two days. (B) Graphic representation of densitometric analysis of nuclear p-NF-κB blot. (C) Graphic representation of densitometric analysis of p-IκBα blot. (D) Immunofluorescence staining of 3T3-L1 pre-adipocyte cells after treatment with 1–20 μM carnosic acid using anti-p-NF-κB (p65) for staining. Data are presented as averages of three independent experiments performed in triplicates and expressed as percentages of the value of the control (means ± standard error mean, n = 3). ###: p < 0.001 vs. Undiff. Different corresponding letters indicate significant differences at p < 0.05 using Duncan’s test. (Undiff: undifferentiated, which was not treated with the MDI hormone mixture; C: control, which was treated with MDI hormone mixture; DAPI: 4′,6-diamidino-2-phenylindole).