Figure 6.

Effects of carnosic acid on translation and nuclear translocation of Nrf2 during MDI-induced differentiation of 3T3-L1 pre-adipocytes to adipocytes for two days. (A) Translation of Nrf2 by Western blotting analysis after differentiation for two days. (B) Graphic representation of densitometric analysis of Nrf2 blot. (C) Immunofluorescence staining of 3T3-L1 pre-adipocyte cells subjected to 1–20 μM carnosic acid using anti-Nrf2. Data are presented as averages of three independent experiments performed in triplicates and expressed as percentages of the value of the control (means ± standard error mean, n = 3). ^###: p < 0.001 vs. Undiff. Different corresponding letters indicate significant differences by Student’s t-test. (Undiff: undifferentiated, which was not treated with the MDI hormone mixture; C: control, which was treated with the MDI hormone mixture; DAPI: 4′,6-diamidino-2-phenylindole).