Table 3.
MDR-based allele-combination analysis of microRNA polymorphisms in idiopathic RPL patients and controls.
| Allele Combination | Controls | RPL | AOR (95% CI) a | p b | FDR-p c |
|---|---|---|---|---|---|
| 2n = 454 | 2n = 776 | ||||
| n (%) | n (%) | ||||
| miR-604, miR-608, miR-631, miR-938, miR-1302-3 | |||||
| A-C-I-G-C | 103 (22.7) | 233 (30.1) | 1.000 (reference) | ||
| A-C-I-G-T | 15 (3.3) | 10 (1.3) | 0.294 (0.128–0.678) | 0.004 | 0.025 |
| A-C-I-A-C | 5 (1.2) | 0 (0.0) | 0.040 (0.002–0.736) | 0.003 | 0.025 |
| A-C-D-G-C | 6 (1.4) | 7 (1.0) | 0.515 (0.169–1.573) | 0.237 | 0.45 |
| A-G-I-G-C | 119 (26.2) | 230 (29.6) | 0.854 (0.620–1.177) | 0.369 | 0.584 |
| A-G-I-G-T | 5 (1.1) | 13 (1.7) | 1.149 (0.399–3.309) | 1.000 | 1.000 |
| A-G-I-A-C | 0 (0.0) | 5 (0.6) | 4.876 (0.266–89.06) | 0.3278 | 0.566 |
| A-G-I-A-T | 0 (0.0) | 1 (0.1) | 1.33 (0.05–32.94) | 1.000 | 1.000 |
| A-G-D-G-C | 7 (1.4) | 15 (1.9) | 0.947 (0.374–2.393) | 1.000 | 1.000 |
| A-G-D-G-T | 0 (0.0) | 1 (0.1) | 1.33 (0.0536–32.94) | 1.000 | 1.000 |
| G-C-I-G-C | 74 (16.3) | 109 (14) | 0.651 (0.447–0.9479) | 0.026 | 0.081 |
| G-C-I-G-T | 0 (0.0) | 10 (1.3) | 9.308 (0.540–160.5) | 0.036 | 0.086 |
| G-C-I-A-C | 0 (0.0) | 3 (0.3) | 3.103 (0.158–60.66) | 0.556 | 0.704 |
| G-G-D-G-C | 0 (0.0) | 3 (0.3) | 3.103 (0.158–60.66) | 0.556 | 0.704 |
| G-G-I-G-C | 90 (19.9) | 125 (16.2) | 0.614 (0.429–0.8772) | 0.008 | 0.034 |
| G-G-I-G-T | 13 (2.8) | 6 (0.8) | 0.204 (0.075–0.5518) | 0.002 | 0.025 |
| G-G-I-A-C | 4 (0.9) | 0 (0.0) | 0.049 (0.002–0.9238) | 0.009 | 0.034 |
| G-G-I-A-T | 3 (0.5) | 0 (0.0) | 3.103 (0.158–60.66) | 0.556 | 0.704 |
| G-G-D-G-C | 8 (1.9) | 5 (0.7) | 0.276 (0.088–0.8651) | 0.03 | 0.081 |
| G-G-D-G-T | 2 (0.4) | 0 (0.0) | 0.088 (0.004–1.864) | 0.095 | 0.2 |
| miR-604, miR-631, miR-938, miR-1302-3 | |||||
| A-I-G-C | 219 (48.3) | 460 (59.3) | 1.000 (reference) | ||
| A-I-G-T | 22 (4.8) | 24 (3.1) | 0.5194 (0.2849–0.9469) | 0.035 | 0.13 |
| A-I-A-C | 7 (1.6) | 7 (0.9) | 0.4761 (0.1649–1.374) | 0.247 | 0.388 |
| A-I-A-T | 0 (0.0) | 1 (0.1) | 1.43 (0.05797–35.27) | 1.000 | 1.000 |
| A-D-G-C | 12 (2.6) | 22 (2.9) | 0.87289 (0.4241–1.796) | 0.71 | 0.867 |
| A-D-G-T | 0 (0.0) | 1 (0.1) | 1.43 (0.05797–35.27) | 1.000 | 1.000 |
| G-I-G-C | 167 (36.9) | 237 (30.6) | 0.6756 (0.5235–0.8721) | 0.003 | 0.034 |
| G-I-G-T | 11 (2.3) | 15 (1.9) | 0.6492 (0.2933–1.437) | 0.291 | 0.401 |
| G-I-A-C | 2 (0.5) | 1(0.1) | 0.238 (0.02146–2.641) | 0.246 | 0.388 |
| G-I-A-T | 3 (0.6) | 0 (0) | 0.06809 (0.003499–1.3250 | 0.034 | 0.13 |
| G-D-G-C | 9 (2.0) | 8 (1.0) | 0.4232 (0.1610–1.112) | 0.112 | 0.247 |
| G-D-G-T | 2 (0.4) | 0 (0.0) | 0.09533 (0.004554–1.996) | 0.105 | 0.247 |
| miR-604, miR-631, miR-1302-3 | |||||
| A-I-C | 228 (50.1) | 467 (60.2) | 1.000 (reference) | ||
| A-I-T | 21(4.5) | 25 (3.2) | 0.5812 (0.3185–1.061) | 0.078 | 0.161 |
| A-D-C | 12 (2.6) | 22 (2.9) | 0.8951 (0.4352–1.841) | 0.851 | 0.993 |
| A-D-T | 0 (0.0) | 1 (0.1) | 1.466 (0.05945–36.16) | 1.000 | 1.000 |
| G-I-C | 168 (37.1) | 238 (30.6) | 0.6916 (0.5369–0.8909) | 0.005 | 0.035 |
| G-I-T | 14 (3.2) | 15 (2.0) | 0.5231 (0.2482–1.102) | 0.106 | 0.161 |
| G-D-C | 9(2.1) | 8 (1.0) | 0.434 (0.1652–1.140) | 0.115 | 0.161 |
| G-D-T | 2 (0.4) | 0 (0) | 0.09775 (0.004670–2.04) | 0.108 | 0.161 |
| miR-604, miR-1302-3 | |||||
| A-C | 239 (52.7) | 489 (63) | 1.000 (reference) | ||
| A-T | 21 (4.6) | 26 (3.3) | 0.6051 (0.3336–1.098) | 0.110 | 0.110 |
| G-C | 178 (39.1) | 246 (31.7) | 0.6755 (0.5275–0.8650) | 0.002 | 0.007 |
| G-T | 16 (3.6) | 15(2.0) | 0.4582 (0.2227–0.9426) | 0.034 | 0.051 |
Abbreviations: RPL, recurrent pregnancy loss; AOR, adjusted odds ratio; CI, confidence interval; FDR-p, false discovery rate-adjusted p. MDR, multifactor dimensionality reduction. a Adjusted for age. b Fisher’s exact test. c False-positive discovery rate (FDR)-adjusted p-value. Bold numbers indicate significant p-values.