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Bilayer characterization by fluorimetric assays of uncoated and HA-Chol-coated niosomes utilizing two probes: pyrene for microviscosity and polarity assessment and DPH for fluidity evaluation.
| Sample with and without HA-Chol | Fluidity (Anisotropy) | Polarity (I1/I3) | Microviscosity (IE/I3) |
|---|---|---|---|
| S2 | 0.11 | 1.14 | 0.38 |
| S2 (1:1) | 0.24 | 1.16 | 0.30 |
| S3 | 0.14 | 1.12 | 0.74 |
| S3 (1:0.5) | 0.15 | 1.23 | 0.36 |
| S3 (1:1) | 0.21 | 1.22 | 0.33 |
| T | 0.20 | 1.58 | 0.18 |
| T (1:0.5) | 0.27 | 1.15 | 0.17 |
| T (1:1) | 0.26 | 1.12 | 0.29 |
