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. 2021 Jun 5;22(11):6106. doi: 10.3390/ijms22116106

Figure 2.

Figure 2

Detection of promoter activity by dual luciferase reporter assay. (A) Analysis of the activity of the primary PxABCG1 resistance promoter (R2) and the main PxABCG1 susceptibility promoter (wild-type S1/S2). The relative luciferase activity (firefly luciferase activity/Renilla luciferase activity) of different pGL4.10-promoter plasmids was normalized to that of the control pGL4.10 vector. The values shown are the means and the corresponding standard error of the mean (SEM) values for three biological replicates and four technical replicates. The significance of differences was determined by one-way ANOVA with Duncan’s test (p < 0.05). Different letters on the bars indicate significant differences. (B) Activity of progressive 5′ deleted recombinants of the S1 and R2 promoters after shortening from −2313 to −310. Student’s t-test was used for statistical analysis (*, p < 0.05). (C) Activity of progressive 5′ deletion constructs created through shortening of the sequence from −1186 to −806. Student’s t-test was used for statistical analysis (*, p < 0.05).