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. 2021 Jun 5;22(11):6106. doi: 10.3390/ijms22116106

Figure 3.

Figure 3

Analysis of the point mutation between −972 and −891. (A) Nucleotide sequence alignment of the fragment from −972 and −891 in PxABCG1 promoters S1 and R2. (B) Activity of the promoters P(-972/-1)-S1 and P(-972/-1)-R2 with site-directed mutagenesis at M-929. The empty pGL4.10 vector was used as a control. The significance of differences was determined by one-way ANOVA with Duncan’s test (p < 0.05). Different letters on the bars indicate significant differences. (C) Effects of Antp and Dfd on the activity levels of PxABCG1 promoters S1 and R2. The empty pAc5.1 vector was used for controls. The significance of differences was determined by one-way ANOVA with Duncan’s test (p < 0.05). Different letters on the bars indicate significant differences.