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. 2021 Jun 5;22(11):6106. doi: 10.3390/ijms22116106

Figure 5.

Figure 5

Effect of Antp on PxABCG1 expression in vivo. (A) Relative expression of the midgut Antp gene in 4th-instar larvae of the susceptible strain DBM1Ac-S and the resistant strain NIL-R. The RPL32 gene was used as an internal control. The average relative expression level and standard error of the mean (SEM) of three independent replicates are presented. Student’s t-test was used for statistical analysis (p < 0.05). (B) Relative expression of Antp and PxABCG1 in larvae of the susceptible strain DBM1Ac-S at 48 h post injection with buffer, dsEGFP, or dsAntp. The expression levels of Antp or PxABCG1 in the control larvae injected with buffer were set as 1. Different letters among groups indicate statistically significant differences (p < 0.05; Duncan’s test; n = 3). Different letters on the bars indicate significant differences. (C) Relative expression of Antp and PxABCG1 in larvae of the resistant strain NIL-R at 48 h post injection with buffer, dsEGFP, or dsAntp. Different letters on the bars indicate significant differences.