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. 2021 Jun 6;13(11):1883. doi: 10.3390/polym13111883

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Representative images of HUVEC sprouting obtained from MSCs conditioned media cultured in each condition (mGelatin vs monolayer; 0.08 T vs 0.45 T; static vs lower frequency vs higher frequency magnetic field). Scale bar: 100 µm. (b) Number of tubes and number of branch points measured for each analysis condition of the tube formation assay. (c) Representative images of the tube formation controls and respective measurement of tubes number and branch points. Endothelial cell culture media, rich in growth factor supplements, was used as the positive control. MSCs secretome, from MSCs cultured in polystyrene culture plate without magnetic exposure, was used as the negative control. Scale bar: 100 µm. All data were obtained from a pool of three independent MSC donors (A, B, C) and presented as means ± SD. Statistical significance was determined using two-way ANOVA: **p < 0.01 a very significant result, ***p < 0.001 a highly significant result; and ****p < 0.0001 an extremely significant result.

(a) Representative images of HUVEC sprouting obtained from MSCs conditioned media cultured in each condition (mGelatin vs monolayer; 0.08 T vs 0.45 T; static vs lower frequency vs higher frequency magnetic field). Scale bar: 100 µm. (b) Number of tubes and number of branch points measured for each analysis condition of the tube formation assay. (c) Representative images of the tube formation controls and respective measurement of tubes number and branch points. Endothelial cell culture media, rich in growth factor supplements, was used as the positive control. MSCs secretome, from MSCs cultured in polystyrene culture plate without magnetic exposure, was used as the negative control. Scale bar: 100 µm. All data were obtained from a pool of three independent MSC donors (A, B, C) and presented as means ± SD. Statistical significance was determined using two-way ANOVA: **p < 0.01 a very significant result, ***p < 0.001 a highly significant result; and ****p < 0.0001 an extremely significant result.