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. 2021 Jun 14;52:86. doi: 10.1186/s13567-021-00955-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Antiviral effect of EP on PDCoV replication in piglets. A Effect of EP on fecal viral shedding. Fecal samples of the indicated groups were collected from 0 dpi (the day before PDCoV infection) to 4 dpi. The relative viral RNA level in the fecal samples was analyzed by RT-qPCR. B Effect of EP on virus distribution. Different tissue samples of the indicated groups were collected at 5 dpi. The relative viral RNA level in the collected samples was analyzed by RT-qPCR. C Western blot analysis of proteins from jejunal and ileal tissues probed with the anti-PDCoV N antibody. The loading was normalized with β-actin. D Fluorescence imaging of jejunal and ileal tissues. Red fluorescence represents the PDCoV distribution, and blue fluorescence represents the nuclear distribution. Scale bars, 20 μm. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.