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. 2021 Jun 14;52:86. doi: 10.1186/s13567-021-00955-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Effect of EP on PDCoV-induced apoptosis in the small intestine. Intestinal tissue samples of the indicated groups were collected at 5 dpi. A TUNEL assay of paraffin-embedded jejunal and ileal tissues. Green fluorescence represents the TUNEL-positive (apoptotic) cells, and blue fluorescence represents the nuclear distribution. Scale bars, 20 μm. B Western blot analysis of proteins from jejunal and ileal tissues probed with the anti-cleaved caspase-3, anti-caspase-3, anti-Bax and anti-Bcl-2 antibody. C–F. Results are presented as the ratio of target protein band intensity to β-actin band intensity. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01.