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. 2021 Jun 14;52:86. doi: 10.1186/s13567-021-00955-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Effect of PDCoV infection on T-lymphocyte subpopulations in the peripheral blood. Peripheral blood samples of the indicated groups were collected at 5 dpi. Left, representative flow cytometry dot plots show the percentage of CD4⁺ T cells within the peripheral blood lymphocytes (A), the percentage of CD4⁺ IFNγ⁺ T cells (B) and CD4⁺ T-bet⁺ T cells (C) within the peripheral blood T cells, and the percentage of CD4⁺ T-bet⁺ IFNγ⁺ T cells within the peripheral blood CD4⁺ T cells (D) in the indicated piglets. Right, flow cytometry analysis of the percentage of CD4⁺ T cells within the peripheral blood lymphocytes (A), the percentage of CD4⁺ IFNγ⁺ T cells (B) and CD4⁺ T-bet⁺ T cells (C) within the peripheral blood T cells, and the percentage of CD4⁺ T-bet⁺ IFNγ⁺ T cells within the peripheral blood CD4⁺ T cells (D) in the indicated piglets. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01.