Table 1.
Sequences of the primers used for RT-qPCR.
| Primers name | Directiona | Sequence (5′ → 3′) |
|---|---|---|
| PDCoV | F | ACCTGTTTCCTTCGCCTTGA |
| R | ACAGCGTCTGGTTGAGTTCC | |
| IL-1β | F | AACGTGCAGTCTATGGAGT |
| R | GAACACCACTTCTCTCTTCA | |
| IL-6 | F | CTGGCAGAAAACA ACCTGAACC |
| R | TGATTCTCATCAAGCAGGTCTCC | |
| IL-12 | F | CGTGCCTCGGGCAATTATA |
| R | CGCAGGTGAGGTCGCTAGTT | |
| TNF-α | F | AACCTCAGATAAGCCCGTCG |
| R | ACCACCAGCTGGTTGTCTTT | |
| IFN-α | F | TCTCATGCACCAGAGCCA |
| R | CCTGGACCACAGAAGGGA | |
| IFN-β | F | AGTGCATCCTCCAAATCGCT |
| R | GCTCATGGAAAGAGCTGTGGT | |
| β-actin | F | TGACTGACTACCTCATGAAGATCC |
| R | TCTCCTTAATGTCACGCACGATT |
aF = forward; R = reverse.