. 2021 May 14;12(6):899–906. doi: 10.1021/acsmedchemlett.0c00682

Table 1. Dissociation Constants (K_D, nM) Measured for Pre-miR-21 Binding Alone or in the Presence of 100 equiv Excess of tRNA (K_D′, nM) or 100 equiv Excess of DNA Duplex (K_D″, nM).

entry	ID	K_D [nM]^a pre-miR-21	K_D′ [nM] (tRNA competition)	K_D′/K_D	K_D″ [nM] (DNA competition)	K_D″/K_D
1	neomycin	1390 ± 126	1415 ± 110	1.0	1950 ± 20.2	1.4
2	1	128 ± 12	134 ± 14	1.0	171 ± 16	1.3
3	6a	133 ± 20	151 ± 13	1.1	187 ± 24	1.4
4	6a′	128 ± 7.0	170 ± 48	1.3	261 ± 60	2.0
5	6b	50.7 ± 7.4	51.0 ± 8.7	1.0	95.7 ± 22	1.9
6	6b′	348 ± 34	405 ± 138	1.2	877 ± 79	2.5
7	6c	50.3 ± 11	50.4 ± 7.0	1.0	80.9 ± 3.2	1.6
8	6c′	162 ± 40	168 ± 10	1.0	274 ± 30	1.7
9	15	641 ± 165	807 ± 270	1.3	1459 ± 70	2.3

Binding studies were performed on 5′-FAM-pre-miR-21 in buffer A (20 mm Tris–HCl (pH 7.4), 12 mm NaCl, 2.5 mm MgCl₂, and 1 mm DTT).

Table 1. Dissociation Constants (KD, nM) Measured for Pre-miR-21 Binding Alone or in the Presence of 100 equiv Excess of tRNA (KD′, nM) or 100 equiv Excess of DNA Duplex (KD″, nM).