FIGURE 4.

DDX10 promotes the proliferation of A549 cells via IMP4. (a) Gene interaction network of DDX10 was generated using the geneMANIA tool (http://www.genemania.org). The thickness of the line indicates the extent of the relationship between two genes. (b) Correlation between DDX10 and IMP4 in LUSC and LUAD. Expression levels are derived from the TCGA mRNA database, and test statistics from Pearson product–moment correlation. (c) Relative DDX10 mRNA expression was detected by real‐time PCR in normal or tumor tissue. (d–e) Relative IMP4 mRNA expression was detected by real‐time PCR in DDX10 knockdown or overexpressed A549 cells. A549 cells were transfected by shCtrl, shDDX10 or shDDX10 + IMP4. (f) Cell viability, (g) apoptosis, and (h) cell cycle of A549 were measured by MTT assay and flow cytometry, respectively. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001