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. 2021 Jun 3;118(23):e2024785118. doi: 10.1073/pnas.2024785118

Fig. 1.

Fig. 1.

Rhox10 represses LINE1 transposition in the male germline. (A) ddPCR analysis of SN1 LINE1 copy number in adult tissues from SN1 reporter mice mated with Rhox cluster (c) cKO (Rhox-cfl/Y;Vasa-Cre;SN1+/−), Rhox10-null (Rhox10-/Y;SN1+/−) (KO), or WT (Vasa-Cre;SN1+/−) mice (n = 6). *P < 0.05. (B) SN1 LINE1 copy number in testes from mice of the ages indicated (adult testis is the same as in A). The genotypes and analysis are described in A (n = 3). *P < 0.05. (C) Luciferase analysis of LINE1 transposition activity in the GC1 spermatogonia cell line cotransfected with the mouse ORFeus LINE1 reporter and a Rhox10-expression vector or an empty expression vector (n = 3). *P < 0.05. (D) qPCR analysis of testes from E16.5 Rhox10-null (KO) and littermate WT mice. The primers used are specific for the indicated LINE1 subfamilies (n = 3). *P < 0.05. (E) Western blot analysis of LINE1 ORF1p protein expression in testes lysates from Rhox10-null (KO) and littermate WT mice of the indicated ages. (Right) ORF1p quantification when normalized to GAPDH (n = 3). *P < 0.05. (F) Immunofluorescence analysis of ORF1p (green) in Rhox10-null (KO) and littermate WT testes from E16.5 mice. The sections were costained with an antibody against TRA98 to detect all germ cells (red) and DAPI to detect all cells (blue). n = 3.

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