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. 2021 May 31;12:653272. doi: 10.3389/fphar.2021.653272

FIGURE 2.

FIGURE 2

Effect of ASIC1a-siRNA transfection and PcTX-1 on ASIC1a, α-SMA, and collagen-Ι expression in HSC-T6 cells (A) Western blotting analysis and densitometric quantification of ASIC1a protein levels in HSCs after transfection of ASIC1a-siRNA; (B) mRNA levels of ASIC1a in HSCs after transfection of ASIC1a-siRNA (C) Expression of the fibrotic proteins in HSCs after transfection of ASIC1a-siRNA; (D) mRNA levels of α-SMA and Collagen-I in HSCs after transfection of ASIC1a-siRNA (E) Western blotting analysis and densitometric quantification of α-SMA, and Collagen-I protein levels in HSCs under PcTX-1; (F) mRNA levels of α-SMA, and Collagen-I in HSCs treated with PcTX-1 (G) Levels of IL-6 and TNF-α in cell medium after treatment with PcTX-1; (H) Levels of IL-6 and TNF-α in the cell medium after transfection with ASIC1A-siRNA. Statistical analyses were performed using t-test. Data are expressed as the mean ± SEM (n = 4). * p < 0.05, **p < 0.01 vs. Control group; # p < 0.05, ## p < 0.01 vs. CuSO4 group.