FIGURE 3.

The effect of regulating the expression of ASIC1a on ERS in copper-treated HSC-T6 cells (A) Western blotting analysis and densitometric quantification of GRP78, and XBP1 protein levels in HSCs treated with PcTX-1; (B) mRNA levels of GRP78, and XBP1 in HSCs treated with PcTX-1 (C) Western blotting analysis and densitometric quantification of GRP78, XBP1 protein levels in HSCs transfected with ASIC1a-siRNA; (D) mRNA levels of GRP78, XBP1 in HSCs transfected with ASIC1a-siRNA. Statistical analyses were performed using t-test. Data are expressed as the mean ± SEM (n = 4). ^* p < 0.05, **p < 0.01 vs. Control group; ^# p < 0.05, ^## p < 0.01 vs. CuSO₄ group.