FIGURE 5.

ASIC1a is involved in regulating the phosphorylation of P38MAPK under copper accumulation conditions (A) Protein expression of phosphorylated P38 MAPK in copper-treated HSC-T6 cells. Data are expressed as the mean ± SEM (n = 6). *p < 0.05, **p < 0.01 vs. Control group (B) Copper-induced changes in the level of phosphorylated P38MAPK in HSCs transfected with ASIC1a-siRNA. Data are expressed as the mean ± SEM (n = 4). ^* p < 0.05, **p < 0.01 vs. Control group; ^# p < 0.05, ^## p < 0.01 vs. CuSO₄ group (C) Immunofluorescence detection of copper-induced phosphorylation of P38MAPK in hepatic stellate cells treated with the P38 MAPK pathway inhibitor SB203580. (magnification 200×) (D) Immunofluorescence detection of copper-induced P38 MAPK phosphorylation in hepatic stellate cells treated with the P38 MAPK pathway agonist dehydrocorydaline (PMA) (magnification 200×).