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. 2021 May 31;12:653272. doi: 10.3389/fphar.2021.653272

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Copyright © 2021 Kong, Huang, Luan, Liu, Ye and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Observation of pathological changes and α-SMA expression induced by Amiloride and D-penicillamine in the livers of copper-treated rats (A) Histopathological results of liver tissues of rats in each group (scale bar, 50 µM) (B) Timm’s copper staining was used to observe the deposition of copper particles in the liver tissues of rats in each group (scale bar, 50 µM) (C) The immunohistochemical method was used to detect α-SMA protein expression in liver tissues of rats in each group (scale bar, 50 µM).