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. 2021 May 31;12:653272. doi: 10.3389/fphar.2021.653272

TABLE 1.

Primer sequences of several genes for real-time PCR.

Gene Forward primer Reverse primer
β-actin 5′-GAG​CGC​AAG​TAC​TCT​GTG​TG-3՛ 5՛-CCT​GCT​TGC​TGA​TCC​ACA​TC-3՛
ASIC1a 5՛-CGG​CTG​AAG​ACC​ATG​AAA​GG-3՛ 5՛-AAG​GAT​GTC​TCG​TCG​GTC​TC-3՛
α-SMA 5՛-GAG​GGA​TCC​TGA​CCC​TGA​AG-3՛ 5՛-CCA​CGC​GAA​GCT​CGT​TAT​AG-3՛
Collagen-Ι 5՛-ACC​TCA​GGG​TAT​TGC​TGG​AC-3՛ 5՛-GAC​CAG​GGA​AGC​CTC​TTT​CT-3՛
GRP78 5′-CTG​TCA​GCA​GGA​CAT​CAA​GTT​C-3’ 5′-TGT​TTG​CCC​ACC​TCC​ATT​ATC​A-3′
XBP1 5′-TCC​GCA​GCA​CTC​AGA​CTA​CG​-3′ 5′-GGC​AAC​AGC​GTC​AGA​ATC​CA​-3′
CCS 5՛-ACA​GCT​GAC​CCC​TGA​GCG-3՛ 5՛-ACA​GAG​CCA​AGG​TGA​GGT​C-3՛
ATP7B 5՛-GCC​AGC​ATT​GCA​GAA​GGA​AAG-3՛ 5՛-TGA​TAA​GTG​ATG​ACG​GCC​TCT-3՛