FIGURE 1.

Analyses of mechanical straining in the stretching device: (A) Schematic overview of mechanical stretching device. The motor (1) generates a torque, which is transmitted to the rotation axis (2) and then to the eccentric disk (3), which ensures that the flexible silicone dish (4) is stretched. The stretching device also allows to stretch small and large silicon dishes (red). (B) Speckled base of the silicon dish (left) and light microscopic evaluation of speckled base of silicon dish (right). (C) Exemplary results after speckle analysis of the base at 10, 5, and 1% strain showing strain distribution across the cell dish area. The dashed boxes show the approximate areas where the images were taken for analysis of cell orientation and YAP nuclear translocation.