Fig. 4.

Labeling of endogenous proteins with sfCherry2_1–10/mTagBFP2_11. (A) A fluorescence image of the split FP complementation from an mTagBFP2₁₁-tagged CASK line crossed with a panneuronal expression line of sfCherry2_1–10. (B) A fluorescence image for the localization of EGFP-tagged CASK. (C–E) Representative images of different mTagBFP2_{11 × 7} protein trap lines. We crossed these mTagBFP2_{11 × 7} lines with a panneuronal line of sfCherry2_1–10. (A, C, and E) The images were taken with the same acquisition settings. (F) Quantification of relative fluorescence intensity of CASK labeled with mTagBFP2_{11 × 1} or mTagBFP2_{11 × 7}. n = 4 to 7 larval brains. Error bars are SEM. (G) ORN-specific labeling of CASK in the adult fly brain. The mTagBFP2_{11 × 7} line of CASK was crossed with an ORN-specific expression line of sfCherry2_1–10 and CD4-tandem GFP. (Scale bars, 100 μm.)