Table 2.

ALS profilin 1 variants show increased interaction with formin family proteins

	C71G		M114T		G118V
Gene	FC	SAINT	FC	SAINT	FC	SAINT
DIAPH2	0	0	8.75	0.97	19.86	1
SOWAHC	0	0	11.55	1	9.53	0.89
DIAPH1	0.85	0	6.78	1	7.55	0.7
FMNL1	1.02	0	5.43	0.95	9.94	0.92

All identified PFN1-interacting proteins in ALS-PFN1 variant cell lines were evaluated for enrichment over the PFN1 WT cell line using SAINT. Several formin family proteins (DIAPH1, DIAPH2, FMNL1) are more abundant. All proteins with a SAINT score ≥0.5 versus WT with any variant are shown. Proteins are ranked by maximum SAINT score versus WT, then by average fold change (FC) as calculated by the SAINT algorithm. SAINT scores below the 0.8 interaction threshold are shown in italics.