Fig. 3.

In vivo treatment efficacy, tested in C57BL/6 mice bearing B16F10 tumors. The animals were i.t. injected with PPF NPs and irradiated with a 671-nm laser at 4 h (PPF + IR). A total of three doses of treatment were given 2 days apart. PBS, NLG919@PLGA NPs in the absence of irradiation (NLG919@PLGA), and ZnF₁₆Pc@FRT plus irradiation (ZnF₁₆Pc@FRT + IR) were tested for comparison (n = 8–10). a Relative tumor volume change. Values are presented as means ± SEM. * p < 0.05, ** p < 0.01. b Animal survival. c Relative volume change for individual tumors from different treatment groups. d H&E and Ki-67 staining with tumor samples taken from all treatment groups. Scale bars, 100 μm