. 2021 Jun 14;21:312. doi: 10.1186/s12935-021-02015-6

Table 1.

The primer sequences which used in RT-PCR and quantitative real-time PCR

Target Transcript	Primer type	Sequence (5ʹ→3ʹ)
hsa_circ_0044234	Forward	TTGTGTTGATCTCAGCAAGCTT
hsa_circ_0044234	Divergent reverse	CTGAGTGTACAATATACATGTCCCA
miR-135b-5p	Stem loop RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAT
	Forward	CTCGTATGGCTTTTCATTCCT
	Universal reverse	GTGCAGGGTCCGAGGT
RNU44	Stem loop RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTCAGTT
RNU44	Forward	GAATGCTGACTGAACATGAAGGTC
GATA3	Forward	CAAGCTTCACAATATTAACAGACCC
GATA3	Reverse	GGGTTAAACGAGCTGTTCTTG
PUM1	Forward	AGTGGGGGACTAGGCGTTAG
PUM1	Reverse	GTTTTCATCACTGTCTGCATCC