Fig. 2.

Characterization of first-round designs. EM, native-MS, and SAXS experiments are consistent with the formation of intended architectures for four designs: three D₂ designs (A) and one D₃ design (B). Negative-stain 3D reconstructions are overlaid with the design models, whose asymmetric units are colored according to the constituent building blocks (N- and C-terminal oligomers, green; DHR, blue; shared alignment, yellow). Native-MS deconvolutions show the relative abundance of the determined masses, and the peaks are labeled with their assigned oligomeric states. SAXS plots compare the theoretical (cyan) and experimental (black) scattering intensities (log scale) as a function of q as well as radius of gyration (Rg).