Fig. 3.

Characterization of second-round designs. EM, native-MS, and SAXS experiments are consistent with the formation of intended architectures for six designs. Negative-stain 3D reconstructions are overlaid by design models, whose asymmetric units are colored according to the constituent building blocks (N- and C-terminal oligomers, green; DHR, blue; shared alignment, yellow). Native-MS deconvolutions show the relative abundance of the determined masses, and the peaks are labeled with their assigned oligomeric states. SAXS plots compare the theoretical (cyan) and experimental (black) scattering intensities (log scale) as a function of q as well as Rg. Native-MS for D3-19.24 shows a small amount of 12-mer, likely formed through the association of two designed hexamers.