Fig. 2.

2D-HFn has a strong ability to bind to cancer cells. a High expression of integrin α2β1 and TfR1 in U-87MG cells, but not in 22Rv1 cells by western blotting using antibodies against integrin α2, integrin β1 and TfR1. b FITC-labeled 2D-HFn had a stronger cell binding ability to U-87MG cells than FITC-labeled HFn. The mean fluorescence intensity (MFI) of FITC-labeled 2D-HFn on U-87MG cells was approximately 3.6-fold greater than that of FITC-labeled HFn (N = 3; mean ± SEM). c After incubation of FITC-labeled 2D-HFn with U-87MG cells, 2D-HFn bound to the cell surface and entered into the cells. In contrast, few 2D-HFn molecules bound to the cell surface of 22Rv1 cells. Scale bar, 10 μm