Skip to main content
. 2021 Jun 4;118(23):e2103730118. doi: 10.1073/pnas.2103730118

Fig. 4.

Fig. 4.

P4 cell induction in aged CD8+ T cells is attenuated by TCR signal suppression through high CD45RB expression. (A and B) Analysis of CD8+ T cell subsets in PLNs of young (2–3 mo old) (A) or aged (12–15 mo old) (B) OT-1 mice with or without i.v. injection of MC38-OVA cells. Representative plots showing CD44 and CD62L expression on CD8+ T cells and the percentage of each CD8+ T cell subset in the indicated mice 5 d after MC38-OVA injection. (C) The percentages of pZAP70+ CD8+ T cells in PLNs from young or aged OT-1 mice with or without MC38-OVA injection are shown in representative plots. (D) CD45RB expression levels in total CD8+ T cells or each subset from PLNs of young (2–3 mo old) or aged (14–17 mo old) OT-1 mice are shown in representative plots. (E) CD8+ T cells were isolated from PLNs of young or aged OT-1 mice and cultured with or without MMC-treated MC38-OVA cells for 1 d. In the last 1 h, vehicle or CD45 inhibitor (0.05 μM) was added. The graphs show the percentages of p-ZAP-70+ cells relative to all CD8+ T cells. Data are represented as the mean ± SEM (n = 3–6). *P < 0.05; **P < 0.01; n.s., not significant (one-way ANOVA followed by Tukey’s test or two-tailed unpaired Student’s t test).