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. Author manuscript; available in PMC: 2021 Jun 14.
Published in final edited form as: Sci Transl Med. 2019 Mar 6;11(482):eaav1648. doi: 10.1126/scitranslmed.aav1648

Fig. 1. CAR design and transduction.

Fig. 1.

(A) The structural gene for 1D3–28Z.1–3 is shown downstream of the MSGV 5′ long terminal repeat (LTR) and splice acceptor (Ψ). The asterisk indicates that the first and third ITAMs in CD3ζ have tyrosine to alanine replacement mutations. (B) Diagram of CAR protein in the cell membrane showing the extracellular single-chain Fv (scFv) domain and the transmembrane (TM) and signaling domains that combine CD28 and CD3ζ C termini. (C) Steps involved in the isolation, activation, and transduction of CD8+ splenocytes with virus before infusion into a recipient mouse that was irradiated ahead of T cell transfer to achieve transient myeloablation. (D) Timeline of CAR T cell treatment and sample collection in NZB/W and MRL-lpr mice. CFSE, carboxyfluorescein diacetate succinimidyl ester.