Fig. 1.

Differences in cell fate upon p53 restoration. (A) Schematic of p53 restorable allele. A stop cassette flanked by loxP sites prevents expression of p53 until Cre excises the cassette and restores wild-type p53. (B, E, and H) Western blots showing restoration of p53 and p21 induction in all tumor types but cleavage of caspase 3 only in lymphoma. Three independent samples were tested for each tumor type. (C and F) Cell cycle analysis showing reduction or loss of S-phase cells after p53 restoration in lung adenocarcinoma and sarcoma cells. (D and G) p53 restoration in lung adenocarcinoma and sarcoma tumors in vivo results in growth arrest but not clearance. (I) Loss of viability in lymphoma cells after p53 restoration at one timepoint and (J) over time. *P < 0.05, ***P < 0.001, ****P < 0.0001.