Fig. 3.

m⁶A facilitates the cytosolic degradation of processed pseudogenes in the GM12878 cell line. a, b Plot of cumulative fraction of expression for processed (a) and unprocessed (b) pseudogene with high (m⁶A level ≥ 0.6), medium (0.6 > m⁶A level ≥ 0.3), and low (m⁶A level < 0.3) m⁶A levels, respectively. Two-tailed Wilcoxon P values are indicated at the bottom right corner. c Plot of cumulative fraction of expression for processed pseudogenes with high (m⁶A level ≥ 0.6), medium (0.6 > m⁶A level ≥ 0.3), and low (m⁶A level < 0.3) m⁶A levels respectively in METTL3 knockdown cells. Two-tailed Wilcoxon P values are indicated at the bottom right corner. d, e Plot of cumulative fraction of nuclear indexes (ratio of expression between the nucleus and cytosol) for processed (d) and unprocessed (e) pseudogenes with high, medium, and low m⁶A levels, respectively. Two-tailed Wilcoxon P values are indicated at the bottom right corner. f Plot of cumulative fraction of nuclear indexes for processed pseudogenes with high, medium, and low m⁶A levels respectively in METTL3 knockdown cells. Two-tailed Wilcoxon P values are indicated at the bottom right corner. g, h Plot of cumulative fraction of cytosolic (g) and nuclear (h) gene expression for processed pseudogene with high, medium, and low m⁶A levels respectively. Two-tailed Wilcoxon P values are indicated at the bottom right corner. i Plot of cumulative fraction of expression of cognate protein-coding genes for processed pseudogene with high, medium, and low m⁶A levels respectively. Two-tailed Wilcoxon P values are indicated at the top left corner and bottom right corner, respectively