Skip to main content
. 2021 Jun 4;118(23):e2104650118. doi: 10.1073/pnas.2104650118

Fig. 5.

Fig. 5.

Effects of FAM195A knockdown. (A) qRT-PCR analysis showing Fam195a mRNA expression in differentiated adipocytes expressing shRNAs scramble and shRNAs against Fam195a transcripts (ShRNA-2, ShRNA-4, and ShRNA-5). *P < 0.05; **P < 0.01. (B) Western blot analysis showing FAM195A protein levels in differentiated adipocytes expressing shRNAs scramble and shRNAs against Fam195a transcripts. GAPDH was used as a loading control. (C) Oil Red O staining of differentiated adipocytes expressing shRNA scrambled as a control and shRNAs against Fam195a transcripts. (D and E) qRT-PCR of selected genes related to BCAA metabolism (D) and mitochondria respiration (Cox15 and Etfdh) and FA transport (Cpt2; E) in differentiated adipocytes expressing shRNAs scramble and shRNAs against Fam195a transcripts. *P < 0.05; **P < 0.01; ***P < 0.001 and ****P < 0.0001. (F) Western blot analysis showing CPT2 and BCKDHA protein levels in differentiated adipocytes expressing shRNAs scramble and shRNAs against Fam195a transcripts. GAPDH was used as a loading control. Data are represented as mean ± SD (A, D, and E); unpaired t test (A, D, and E). SVF, stromal vascular fraction.