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. 2021 Jun 4;118(23):e2104650118. doi: 10.1073/pnas.2104650118

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Fig. 6. — FAM195A interactome in adipocytes. (A) Western blot analysis showing FAM195A-miniTurbo expression in differentiated adipocytes. FLAG-HA-FAM195A fusion protein was used as a control for antibody specificity; alpha-tubulin was used as loading control. (B) Western blot analysis showing protein biotinylation in the input lysate and precipitation of biotinylated proteins after streptavidin immunoprecipitation. (C) Volcano plot representing the abundance of proteins immunoprecipitated after proximity labeling in two independent experiments performed in differentiated adipocytes. The red dotted square contains protein with normalized abundance >20. (D) GO analysis of top enriched terms observed from C. (E) SMART protein domain analysis from C. (F) Western blot analysis of differentiated adipocytes coinfected with FAM195A-miniTurbo and FLAG-HA-Znf281, FLAG-HA-Junb, and FLAG-HA-Fip1L1. (G) Western blot analysis of differentiated adipocytes coinfected with FAM195A-miniTurbo and FLAG-HA-Safb. BIO+ cells were pulsed with biotin for 2 h, and BIO− cells were left untreated. IP, immunoprecipitation; IB, immunoblot.