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Immunohistochemistry for Collagen 1. Right ventricular tissue from adult wild-type mice WT and EC-SOD transgenic mice (TG), which were exposed to FiO2 10% hypoxia for 21 days (WT) and a room air control group (RA) were treated with Cy3 stain to assess for levels of Collagen 1 (A). The images were analyzed using Fiji image processing software to measure pixel density (B). All experiments n = 5. Data represents mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
