Figure 5.

Central mechanism in miR-29a/b₁ KO mice. (A, B) GnRHR immunoreactivity in pituitary of wild-type and miR-29a/b₁ KO females. The receptor was not detectable on the plasma membrane of control. (immunohistochemical: wild-type: 13.43 ± 0.7927, miR-29a/b₁ KO: 25.47 ± 0.534, p=0.0249; immunofluorescence: wild-type: 37.79 ± 1.858, miR-29a/b₁ KO: 56.64 ± 2.767, p=0.0045, n=3). (C) Serum LH and FSH levels in miR-29a/b₁ KO females and controls following ovariectomy (OVX) and sham-operated controls (Sham). (LH: wild-type: p=0.0401, miR-29a/b₁ KO: p=0.9249; FSH: wild-type: p=0.0016, miR-29a/b₁ KO: p=0.0185, n=6). (D) Expression of Kiss1 and Gnrh1 in hypothalamus (Gnrh1: wild-type: 1 ± 0.1912, miR-29a/b₁ KO: 0.7287 ± 0.06234, p=0.1874, Kiss1: wild-type: 1 ± 0.1305, miR-29a/b₁ KO: 0.8142 ± 0.0757, p=0.8405, n=15). (E) Normal distribution of GnRH neurons in miR-29a/b₁ KO mice compared to control littermates. OVLT, organum vasculosum of the lamina terminalis. Scale bars, 200μm. (wild-type: 9.827 ± 1.547, miR-29a/b₁ KO: 8.597 ± 0.8466, p=0.5238, n=3). *p < 0.05, **p < 0.01 and ****p < 0.0001.