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. 2021 May 31;12:698275. doi: 10.3389/fphar.2021.698275

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Copyright © 2021 Feng, Feng, Zhang, Li and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Matrix stiffness changes the morphology of pericytes isolated from mouse lung vasculatures. (A) Representative immunofluorescence images of pericytes that are NG2 and αSMA positive but FSP1 negative. Cell nuclei were stained with DAPI. Scale bar is 200 um. (B) Morphology changes of pericytes cultured on different matrices that vary in stiffness. Scale bar is 20 um. (C) Statistical analysis of the spreading areas of pericytes on different matrices. (D) The distribution of the major axis of pericytes vs. their minor axis on different matrices.