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. 2021 Jun 10;9(6):e002508. doi: 10.1136/jitc-2021-002508

Figure 5.

Figure 5

T cell-recruitment chemokines expression is associated with NF-κB signaling after MSNs treatment. (A) Correlation among the expression of T cell-recruitment chemokines across all B2m-sgRNA B16F10 tumor tissues. (B) Through transcriptomic analysis, all genes with significantly positive correlation with CCL5 were identified. (C) Cell-specific enrichment of CCL5-correlated genes. (D) Transcriptional regulatory enrichment of CCL5-correlated genes. (E) Top 10 signaling pathways by KEGG pathway enrichment of Ccl5-correlated genes. (F) In MSNs-treated tumors, the expression of genes in the top 10 Ccl5-related signaling pathways was presented by heatmap. (G) In tumor tissues, the activation changes of NF-κB, TNF and TLR signaling pathways in αPD-1 plus MSNs groups compared with αPD-1 alone were revealed by GSEA. (H) In tumor tissues after indicated treatments, the levels of proteins in TLR4, TNFR and NF-κB signaling were determined by Western blot. GSEA, Gene Set Enrichment Analysis; MSNs, mesoporous silica nanoparticles; PD-1, programmed cell death protein 1; TLR, Toll-like receptor; TNF, tumor necrosis factor.