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. 2021 May 14;3(2):fcab104. doi: 10.1093/braincomms/fcab104

Figure 1.

Figure 1

Immunostaining of phospho-alpha synuclein (p-aSyn, S129) in dorsal root ganglia (DRG, L4) and spinal grey matter of M83+\+ mice. (A) Representative images showing p-aSyn (S129) IHC in L4 DRG: PBS injected- dpi 45 and PFF aSyn injected- dpi 14, dpi 21 and dpi 45 (PBS, phosphate buffered saline; PFF, pre-formed alpha synuclein- aSyn fibrils; dpi, days post-injection; 40× magnified views, scale bar = 50 µm). (B) Representative images showing p-aSyn (S129) IHC in lumbar grey matter of PFF aSyn injected cohorts (dpi, days post-injection; DH, dorsal horn; IG, intermediate grey; VH, ventral horn; 40× magnified views, scale bar = 50 µm). Yellow arrows point to select instances of neuritic aSyn pathology. Also see Supplementary Fig. 1A, for representative images from PBS injected cohort- dpi 45. (C) Representative images showing p-aSyn (S129) IHC in thoracic grey matter of PFF aSyn injected cohorts (dpi, days post-injection; DH, dorsal horn; IG, intermediate grey; VH, ventral horn; 40× magnified views, scale bar = 50 µm). Primary antibody in A–C: p-aSyn (S129)- 11A5.