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. 2021 May 14;3(2):fcab104. doi: 10.1093/braincomms/fcab104

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© The Author(s) (2021). Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Composite schematic depiction of the early stage phospho-alpha synuclein (p-aSyn, S129) immunodetection in the CNS of PFF injected M83^+/+ mice. Average number of p-aSyn (S129) immunopositive cells in the regions is indicated by the red circles (small circle, 1–10 cells/mm²; medium circle, 10–30 cells/mm²; large circle, >30 positive cells/mm²). Regions shown: (Spinal cord) ventral horn, intermediate grey, dorsal horn; (Medulla oblangata) MdV, medullary reticular nuclei; (Pons) gigantocellualr nuclei, vestibular nuclei, cerebellar nuclei; (Midbrain) periaqueductal grey, ed nucleus; (Forebrain) motor cortex [dpi, days post-intramuscular injection with preformed fibrililar (PFF) aSyn; Structures are not drawn to scale]. Also see Figs. 1–3 and Supplementary Fig. 3.